Increase Yield and Consistency
New research shows that active control of dissolved CO₂ in bioproduction increases product yield and improves consistency of process scale-up and scale-down. Traditional electrochemical sensors indirectly measure DCO₂, are maintenance intensive, and are prone to measurement drift. CO₂NTROL is a Solid State Sensor that directly measures DCO₂ and provides maintenance free, real-time, and in-line control of this new critical process parameter.
Automated Control of DCO₂ Enables:
While the small surface area to volume ratio of a typical R&D reactor means that CO₂ accumulation is minimal. Real-time control of CO₂ is critical to define the optimal set-point and optimize product yield.
Mimicking sparging and stripping strategies optimized for R&D will result in different conditions as the surface to volume ratio decreases. Active control of an optimal DCO₂ profile ensures consistency across scales.
For existing processes that were developed without DCO₂ control, the efficiency of scale-down studies can be improved by actively controlling DCO₂ to mimic the profile seen in the production reactor.
Impact of CO₂ on Process Performance
|▯ Excessive Accumulation|
During a process excess CO₂ accumulation is common as cell concentration increases and more metabolic CO₂ is produced. This accumulation reduces intracellular pH resulting in slower enzymatic activity or delayed lactate shift. The outcome is lower production quantity and quality.
|▯ Excessive Removal|
Aggressive aeration and mixing can prevent detrimental accumulation of CO₂. However, too little CO₂ can slow cell growth, metabolism, and productivity by starving cells of CO₂ needed for the formation of metabolic intermediates. Excessive removal can also reduce buffer capacity in bicarbonate systems.
|▯ Uncontrolled CO₂ range|
Media addition and process adjustments can result in large swings in dissolved CO₂ that may go unnoticed with infrequent offline monitoring and adjustment. Continuous inline control at the optimal level yields increased viable cell density, production phase duration, and titer.
No Ammonia Fouling
Seamless control of all Arc products from a PC or mobile device. CO2NTROL pairs with the newest ArcAir software to automatically store all sensor activities, diagnostic data, and health indicators throughout the life of the sensor. ArcAir is ready for GMP with Part 11 and Annex 11 compliance.
An optional Bluetooth adapter and ArcAir software enable wireless calibration, configuration, and monitoring of all sensor details.
Arc sensors save space and cost with their integrated micro-transmitters. Calibration data is stored in the sensor head enabling high quality lab calibration.
The CO2NTROL sensor transmits process data through a hardwired connection. Communication options include modbus RTU and 4-20 mA, with additional compatibility for OPC, Profibus, and Foundation Fieldbus coming soon.
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