Cell Density Sensors
The Power of In-Line Cell Density Measurement
On-line, or in-situ, measurement is the pinnacle of process monitoring and necessary for true process control as evidenced with control of pH and dissolved oxygen, common for most biological processes. In fact, many parameters can be monitored but those directly related to cell physiology are typically time consuming off-line measurements that provide only a reactionary window into the past. Incyte Arc and Dencytee sensors provide a means for directly measuring viable cell density and total cell density in real-time, meeting the increasing need for PAT in the biopharmaceutical industry.
Viable Cell Density Sensors
Dencytee Total Cell Density Sensors
How Does Cell Density Measurement Work?
Many parameters like pH and dissolved oxygen can be monitored in-line but those directly related to cell physiology are typically time consuming offline measurements that require many manual steps. Total and Viable Cell Density measurement is now possible in-line with a new breed of sensors. Learn more about saving time and effort and increasing productivity and process understanding with in-line cell density sensors in the video below.
Will Hamilton Cell Density Sensors Work With My Process?
Hamilton cell density sensors are compatible with many types of cell lines and microbial strains, process types, and industries. Read more below or download our Cell Density Applications eBook to see real data with different cell lines and process types.
Specific Applications
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App Note - Real-time cell density measurement for PAT applications
An application highlighting the use of Incyte and Dencytee in a PAT approach to monoclonal antibody (mAb) production. -
App Note - Xylitol fermentation
Learn how Hamilton pH, DO, and cell density sensor technology helps the Budapest University of Technology and Economics work with different yeast strains to optimize xylitol yield. -
App Note - Real-Time VCD Monitoring of Moss for Therapeutic Protein Production
In-line Measurement of Viable Cell Density in a Photobioreactor -
App Note - Real-time total cell density measurement of yeast fermentations
Dencytee is used to optimize fermentation conditions for yeast processing.
Resources
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Brochure - Arc Intelligent Sensors
Arc Intelligent sensors offer a new way to connect to your process control system. -
Support Resource - Incyte Data Analysis Macro
Import offline data from the offline data worksheet and Incyte data into this macro for automatic generation of data analysis graphs. -
Support Resource - Incyte Protocol for Offline Measurement Correlation (Demo Protocol)
This guide details how to obtain the relevant offline measurements and online Incyte (permittivity) data of viable cell density to establish a correlation.
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Will Incyte work with my cell line?
Incyte has been shown to work with cell lines ranging from cell culture to microbial fermentation. We have compiled a current list of known applications in our cell density applications ebook. Download a copy to learn more. -
How does Incyte measure viable cell density?
Incyte is a capacitance-based sensor responsive only to living cells. -
What is the effect of gas bubbles on the Incyte measurement?
Gas bubbles have two main impacts: 1. Decrease in the signal intensity, since the Sensor is not measuring in air 2. Increase of background noise
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Cell Density Knowledge Base Articles
A summary page with links for all Cell Density related articles -
Connecting Cell Density (Pre-amp Versions) to External Systems
This article discusses the various options to output cell density measurement data to external devices such as PLCs and control systems. -
Data Analysis Tools (Cell Density Pre-Amp versions)
Hamilton provides several tools to interpret cell density data and correlate it with offline measurements. Download them here.
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